Micromachined High Aspect Ratio PDMS Elastic Micropost Arrays for Studying Traction Forces of Vascular Smooth Muscle Cells
نویسندگان
چکیده
PDMS micropost arrays were fabricated, characterized, and used to analyze the traction force generated by vascular smooth muscle (VSM) cells. The micropost array was fabricated with diameters between 3-7μm, with edge to edge spacing of 5 and 7m, and height to diameter aspect radio up to 8. The spring constant of micropost used in cell culturing experiment is 20.94nN/m and the smallest traction force exerted from VSM cell is 8.05nN. The force generated by VSM cells cultured on top of micropost was in the nano Newton (N) range and it can be obtained by measuring the micropost deflection using optical microscopy images. The high aspect ratio micropost can be used to measure the traction forces of cells with weaker contractile forces in the nN-pN range.
منابع مشابه
PDMS Elastic Micropost Arrays for Studying Vascular Smooth Muscle Cells.
This paper describes the design, modeling, fabrication and characterization of a micromachined array of high-density 3-dimensional microposts (100×100) made of flexible material (silicone elastomers) for use to measure quantitatively the cellular traction force and contractile events in isolated vascular smooth muscle cells (VSMCs). The micropost array was fabricated with diameters ranged from ...
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